B3GNT2 Knockout cell line(A375)

B3GNT2 Knockout cell line(A375) Order

Catalog#

YKO-H486

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

B3GNT2 Knockout cell line (A375)

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Gene Name

B3GNT2

Gene ID

10678

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Target region

Knockout region: Exon 2 (based on transcript B3GNT2-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:A375			STR Info (Cell bank) Cell Line:A-375 [A375]
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	11	14		11	14
D7S820	9	9		9	9
D16S539	9	9		9	9
VWA	16	17		16	17
TH01	8	8		8	8
AMEL	X	X		X	X
TPOX	8	10		8	10
CSF1PO	11	12		11	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the B3GNT2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A375 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, B3GNT2 gene knockout B3GNT2 Knockout cell line (A375) will be expanded and cryopreserved.

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